Murdoch University WA used 7 male greyhound dogs in an attempt to document particular changes in their urine subsequent to artificially inducing a model of renal (kidney) injury produced by haemorrhage and colloid fluid resuscitation.
The dogs used in the experiment were ‘donated’ to the hospital and scheduled to be used as terminal blood donors.
First, the dogs were fasted for at least 8 hours before the experimental procedure. They were then anaesthetised, intubated, and mechanically ventilated. A cannula was then inserted the cranial vena cava (vein) via the right jugular vein for collecting blood samples and for injecting lithium chloride. The left femoral artery was also cannulated in order to facilitate the removal of blood to generate experimental haemorrhage, as well as to measure mean arterial pressure and lithium chloride voltage for cardiac output calculation. After a femoral nerve block was performed, the femoral artery was surgically exposed and a cannula inserted. A transducer was then positioned at the level of the right atrium (of the heart). A urinary catheter was also inserted into the bladder for collecting urine samples and measuring urine output.
Blood and urine samples were collected throughout the experiment.
Following the above ‘instrumentation’, blood was removed from the dogs via the femoral artery catheted for 60 minutes, causing haemorrhage.
Following haemorrhage, the dogs were infused with a solution (gelatin-based colloid) for 3 hours. This experimental procedure produced acute renal tubular damage in the dogs.
The dogs were then killed via an injection of pentobarbital, and their kidneys removed.
(Davis, J, Raisis, A, Cianciolo, R, Miller, D, Shiel, R, Nabity, M & Hosgood, G 2016. ‘Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs’, Veterinary Anesthesia and Analgesia, 43: 262-270.)
What You Can Do
Click here to sign our petition demanding an end to the use of dogs in experiments.